Rachel L. Markley, Ph.D

Rachel.L.Markley@gmail.com

https://www.linkedin.com/in/rachel-markley-33277819/

RLMarkley.org

(610) 613-1406

Statement of Focus

My research interest is to understand the influence of nutrition on inflammation and resulting pathogenesis. The objective of my doctoral thesis was to determine the effect of the trace element selenium on the outcome of acute bacterial infections, and establish the underlying mechanism. Specifically, I have established that selenoproteins restrict the intracellular replication of the gram-negative bacteria Francisella tularensis by suppression of autophagy in macrophage cells.

 

Education                          

2011-2017                Ph.D. Immunology & Infectious Diseases (IID)

The Pennsylvania State University, University Park, PA

Thesis: “Restriction of intracellular replication of Francisella tularensis by selenoproteins”

Advisor: Girish Kirimanjeswara

2009-2010                MSc.  Biotechnology

The Pennsylvania State University, University Park, PA

Thesis: “Proteomics Profiling of Chlamydia trachomatis serovar D Elementary Bodies”

Advisor: Loida Escote-Carlson

2000-2004                B.S. Biology

Gettysburg College, Gettysburg, PA

Dual Major: Visual Arts

Minor: Philosophy

Thesis: “Allelopathic Effects of Helianthus annuus on Hordeum vulgare”           

Work Experience

2011 –2017               Graduate Assistant, The Pennsylvania State University, University Park, PA

                                    Supervisor: Girish Kirimanjeswara

2010 – 2011              Cooperative learning Experience, Merck & Co. (Vaccines Basic Research), West Point, PA

Supervisor: Eberhard Durr

2004 – 2008              Business Manager, Polymics Ltd., State College, PA

Supervisor: Tim Hsu

2002                           Research Assistant, Gettysburg College, Gettysburg, PA

Supervisor: Steven James

 

Manuscripts

1.     Goralski TD, Dewan KK, Alumasa JN, Avanzato V, Place DE, Markley RL, Katkere B, Rabadi SM, Bakshi CS, Keiler KC, Kirimanjeswara GS. “Inhibitors of Ribosome Rescue Arrest Growth of Francisella tularensis at All Stages of Intracellular Replication.” Antimicrobial agents and chemotherapy vol. 60,6 3276-82. 23 May. 2016, doi:10.1128/AAC.03089-15

2.      Sumner SE, Markley RL, Kirimanjeswara GS “Role of Selenoproteins in Bacterial Pathogenesis” Biological Trace Element Research (manuscript submitted June 2019)

3.     Markley RL, Williamson DR, Katkere B, Dewan KK, Shay AE, Carlson BA, Prabhu KS, and Kirimanjeswara GS“Macrophage selenoproteins restrict intracellular replication of Francisella tularensis” (manuscript in preparation)

4.      Williamson DR, Dewan KD, Patel T, Katkere B, Markley RL, and Kirimanjeswara GS “Intracellular growth of Francisella tularensis requires rgb-P-mediated adaptation to cytosolic pH” (manuscript in preparation)

5.     Katkere B, Markley RL, Williamson DR, Place DP, Shay A, Bradson CL, Prabhu KS, and Kirimanjeswara GS “Regulation of B cell receptor mediated functions by selenium” (manuscript in preparation)

 

Fellowships and Grants

      National Institutes of Health training grant T32AI074551 2013

      Graduate Fellowship, Huck Institute Life Sciences (IID) Aug 2011- Aug 2012

 

Awards

      The American Association of Immunologists (AAI) Travel Award 2017

      Huck Travel Award 2016

      Center for Molecular Immunology and Infectious Disease (CMIID) Travel Award 2016

      The American Society for Cell Biology Travel Award Nov 2016

      College of Agricultural Sciences Travel Grant May 2014

 

Presentations

“Restriction of intracellular replication of Francisella tularensis by selenoproteins” To be presented May 14, 2017, AAI General Meeting, Washington DC

 

“Comprehensive Profiling of the Chlamydia trachomatis serovar D Proteome to Support Subunit Vaccine Target Discovery” Presented May 2011, Merck & Co., West Point, PA

 

Posters

      “Macrophage selenoproteins restrict intracellular replication of Francisella tularensis” Presented by Rachel Markley, ASCB General Meeting, San Francisco, CA, USA; Dec 3-7, 2016.

      “The Selenium Status of Macrophages Influences In Vitro Francisella Pathogenesis” Presented by Rachel Markley, ASM General Meeting, Boston, MA, USA; May17-20, 2014.  The Jackson Laboratory, 55th Annual Short Course on Medical and Experimental Mammalian Genetics, Bar Harbor, ME, USA; July 20 – Aug 2, 2014.  17th Annual Upstate New York Immunology Conference, Bolton Landing, NY, USA; Oct 19 – 21, 2014.

      “Modulation of Tularemia Pathogenesis through Selenium Supplementation” Presented by Rachel Markley, 16th Annual Upstate New York Immunology Conference, Bolton Landing, NY, USA; Oct 20 – 23, 2013.

      “The Role of Selenium in Autophagy: Implications for the Pathogenesis of Tularemia” Presented by Rachel Markley, 32nd Summer Symposium, The Pennsylvania State University, University Park, PA, USA; Jun 4 – 6, 2013.

      “Comparative Proteomic Profiling of two Chlamydia trachomatis serovar D Life Cycle Stages using Label Free Shotgun Proteomics” Presented by Eberhard Durr, 60th ASMS Conference on Mass Spectrometry and Allied Topics, Vancouver, BC, Canada; May 20 - 24, 2012

      “Characterization of Chlamydia trachomatis serovar D (strain UW-3/Cx) elementary bodies by shotgun proteomics" Presented by Rachel Markley, Merck & Co., West Point, PA; Dec 2010

 

 

Mentoring:

      Jennifer Curtis – Erickson Discovery Grant Program recipient

      Shafiq Yasuw Alvarado

      Assisted with Upward Bound Math and Science Summer STEM 2016